edman degradation sentence in Hindi
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- He developed a method for sequencing proteins, the Edman degradation.
- The resulting fragments were then microsequenced by an automated Edman degradation.
- Phenylisothiocyanate, the reagent for the Edman degradation, can also be used.
- Edman degradation is generally not useful to determine the positions of disulfide bridges.
- The amino sequence of the protease inhibitor was determined by using the automatic Edman degradation method.
- An artificial isothiocyanate, phenyl isothiocyanate, is used for amino acid sequencing in the Edman degradation.
- I see some interesting results from the Edman degradation reaction-- how does the sulfide leave the ring?
- An advantage of the Edman degradation is that it only uses 10-100 pico-moles of peptide for the sequencing process.
- The Edman degradation is an alternative method for peptide sequencing that cleaves amino acid residues from the N-terminus of a peptide.
- Researchers are also developing a new method of peptide cleavage based on Edman degradation, a method of sequencing amino acids in a peptide.
- The Edman degradation reaction was automated in 1967 by Edman and Beggs to speed up the process and 100 automated devices were in use worldwide by 1973.
- Because the Edman degradation proceeds from the N-terminus of the protein, it will not work if the N-terminus has been chemically modified ( e . g . by acetylation or formation of Pyroglutamic acid ).
- The primary structure was first determined by Yamasaki and Maekawa, who carried out the experiment using the Edman degradation method for N-terminus sequencing and carboxypeptidase A ( Cpase A ) and triazination methods for C-terminus sequencing.
- Following Sanger's initial report of the reagent, the dinitrofluorobenzene method was widely adopted for studying proteins, until it was superseded by other reagents for terminal analysis ( e . g ., dansyl chloride and later aminopeptidases and carboxypeptidases ) and other general methods for sequence determination ( e . g ., Edman degradation ).
- Historically, short protein sequences ( 10 to 15 residues ) determined by Edman degradation were back-translated into DNA sequences that could be used as probes or primers to isolate molecular clones of the corresponding gene or complementary DNA . The sequence of the cloned DNA was then determined and used to deduce the full amino-acid sequence of the protein.
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